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雌激素(Estrogen)酶聯免疫吸附檢測試劑盒
ELK9031
規格: 價格:
48T ¥1960.00
96T ¥2800.00

Overview 文獻

Product name: Estrogen ELISA Kit
Reactivity: General
Alternative Names: E ; Estrogen
Assay Type: Competitive Inhibition
Sensitivity: 4.15 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.625-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 2h
Research Area: Reproductive science;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Estrogen protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Estrogen. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Estrogen in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (pg/mL) OD Corrected OD
1000.00 0.199
500.00 0.389
250.00 0.593
125.00 0.857
62.50 1.112
31.25 1.579
15.63 1.869
0.00 2.375

精密度

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Estrogen and the recovery rates were calculated by comparing the measured value to the expected amount of Estrogen in samples.
Matrix Recovery range Average
serum(n=5) 89-103% 96%
EDTA plasma(n=5) 83-98% 90%
Heparin plasma(n=5) 81-95% 99%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Estrogen and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 96-104% 87-98% 89-102% 86-97%
EDTA plasma(n=5) 95-103% 80-96% 87-98% 85-92%
Heparin plasma(n=5) 87-98% 85-96% 92-102% 81-90%
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