綿羊孕酮(PG)酶聯(lián)免疫吸附檢測試劑盒-科鹿（武漢）生物科技有限責(zé)任公司

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綿羊孕酮(PG)酶聯(lián)免疫吸附檢測試劑盒

ELK8876

規(guī)格：	價(jià)格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Sheep PG(Progesterone) ELISA Kit
Reactivity:	Sheep
Alternative Names:	P4; Pregn-4-Ene-3,20-Dione
Assay Type:	Competitive Inhibition
Sensitivity:	0.47 ng/mL
Standard:	100 ng/mL
Detection Range:	1.57-100 ng/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	2h
Research Area:	Endocrinology;Reproductive science;Hormone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sheep PG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep PG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep PG in the samples is then determined by comparing the OD of the samples to the standard curve.

Molecular Biology Reports

Association of birth type and LHX4 gene polymorphism with reproductive hormones, growth hormone, and prolactin in Awassi ewes

日期：2023-02-14 IF：2.742

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
100.00	0.227
50.00	0.375
25.00	0.557
12.50	0.831
6.25	1.119
3.13	1.511
1.57	1.762
0.00	2.325

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant PG and the recovery rates were calculated by comparing the measured value to the expected amount of PG in samples.

Matrix	Recovery range	Average
serum(n=5)	78-92%	85%
EDTA plasma(n=5)	85-99%	92%
Heparin plasma(n=5)	87-99%	91%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.