綿羊生長(zhǎng)激素(GH)酶聯(lián)免疫吸附檢測(cè)試劑盒-科鹿（武漢）生物科技有限責(zé)任公司

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綿羊生長(zhǎng)激素(GH)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK8850

規(guī)格：	價(jià)格：
48T	￥2240.00
96T	￥3200.00

下載說(shuō)明 ①

Overview 文獻(xiàn)

Product name:	Sheep GH(growth hormone) ELISA Kit
Reactivity:	Sheep
Alternative Names:	GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary Growth Hormone; Growth Hormone, Normal
Assay Type:	Sandwich
Sensitivity:	0.139 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	Endocrinology;Neuro science;Hormone metabolism;
Uniprot ID:	P67930
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep GH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep GH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep GH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep GH in the samples is then determined by comparing the OD of the samples to the standard curve.

Molecular Biology Reports

Association of birth type and LHX4 gene polymorphism with reproductive hormones, growth hormone, and prolactin in Awassi ewes

日期：2023-02-14 IF：2.742
Molecular Biology Reports

Association of birth type and LHX4 gene polymorphism with reproductive hormones, growth hormone, and prolactin in Awassi ewes

日期：2023-02-14 IF：2.74

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
20.00	2.136	2.053
10.00	1.536	1.453
5.00	1.183	1.100
2.50	0.864	0.781
1.25	0.527	0.444
0.63	0.377	0.294
0.32	0.232	0.149
0.00	0.083	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant GH and the recovery rates were calculated by comparing the measured value to the expected amount of GH in samples.

Matrix	Recovery range	Average
serum(n=5)	85-98%	91%
EDTA plasma(n=5)	81-95%	88%
Heparin plasma(n=5)	92-107%	99%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.