犬血管緊張素Ⅱ(AngII)酶聯(lián)免疫吸附檢測試劑盒-科鹿（武漢）生物科技有限責(zé)任公司

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犬血管緊張素Ⅱ(AngII)酶聯(lián)免疫吸附檢測試劑盒

ELK8598

規(guī)格：	價(jià)格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview 文獻(xiàn)

Product name:	Dog AngII(Angiotensin II) ELISA Kit
Reactivity:	Dog
Alternative Names:	Ang-II; Angiotensin-2
Assay Type:	Sandwich
Sensitivity:	1.32 pg/mL
Standard:	200 pg/mL
Detection Range:	3.13-200 pg/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length:	3.5h
Research Area:	Endocrinology;Cardiovascular biology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog AngII. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog AngII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog AngII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog AngII in the samples is then determined by comparing the OD of the samples to the standard curve.

International Journal of Medical Sciences

Blockade of SK4 channels suppresses atrial fibrillation by attenuating atrial fibrosis in canines with prolonged atrial pacing

日期：2022-11-23 IF：3.642
Tissue & Cell

Regulation of endothelial cells on the osteogenic ability of bone marrow mesenchymal stem cells in peri-implantitis

日期：2023-02-20 IF：2.586

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
200.00	2.043	1.955
100.00	1.577	1.489
50.00	1.217	1.129
25.00	0.765	0.677
12.50	0.493	0.405
6.25	0.342	0.254
3.13	0.263	0.175
0.00	0.088	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant AngII and the recovery rates were calculated by comparing the measured value to the expected amount of AngII in samples.

Matrix	Recovery range	Average
serum(n=5)	82-94%	88%
EDTA plasma(n=5)	85-97%	91%
Heparin plasma(n=5)	81-93%	87%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AngII and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.