<b id="37kmu"></b>

<b id="37kmu"></b>

<b id="37kmu"></b>

產品中心

犬白介素1β(IL1b)酶聯免疫吸附檢測試劑盒
ELK1296
規格: 價格:
48T ¥1680.00
96T ¥2400.00

Overview 文獻

Product name: Dog IL1b(Interleukin 1 Beta) ELISA Kit
Reactivity: Dog
Alternative Names: IL-1B;IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin
Assay Type: Sandwich
Sensitivity: 3.1 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;
Uniprot ID: Q28292
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (pg/mL) OD Corrected OD
500.00 1.908 1.800
250.00 1.628 1.520
125.00 1.027 0.919
62.50 0.752 0.644
31.25 0.438 0.330
15.63 0.262 0.154
7.82 0.216 0.108
0.00 0.108 0.000

精密度

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IL1b and the recovery rates were calculated by comparing the measured value to the expected amount of IL1b in samples.
Matrix Recovery range Average
serum(n=5) 77-95% 86%
EDTA plasma(n=5) 92-106% 99%
Heparin plasma(n=5) 88-103% 95%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL1b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 91-103% 86-92% 88-97% 89-97%
EDTA plasma(n=5) 79-94% 87-96% 78-93% 82-90%
Heparin plasma(n=5) 95-102% 89-96% 87-98% 85-93%
關閉

在線咨詢

Online consultation

  • 在線咨詢
  • 技術支持

關注微信公眾號

微信掃一掃立即咨詢

微信掃一掃立即咨詢

關閉
人妻在线欧美视频|中文字幕在线人成视频欧美|欧美一区亚洲国产粉|亚洲全部无码中文字幕
<b id="37kmu"></b>

<b id="37kmu"></b>

<b id="37kmu"></b>