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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
500.00 | 1.908 | 1.800 |
250.00 | 1.628 | 1.520 |
125.00 | 1.027 | 0.919 |
62.50 | 0.752 | 0.644 |
31.25 | 0.438 | 0.330 |
15.63 | 0.262 | 0.154 |
7.82 | 0.216 | 0.108 |
0.00 | 0.108 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 77-95% | 86% |
EDTA plasma(n=5) | 92-106% | 99% |
Heparin plasma(n=5) | 88-103% | 95% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 91-103% | 86-92% | 88-97% | 89-97% |
EDTA plasma(n=5) | 79-94% | 87-96% | 78-93% | 82-90% |
Heparin plasma(n=5) | 95-102% | 89-96% | 87-98% | 85-93% |
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